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Halo tag vector

WebDescription All vectors are compatible with Promega Flexi Vector System, directional cloning method for protein-coding sequences Vectors designated “pFC” are configured to append HaloTag 7 to carboxy-terminus of … WebVector backbone pcDNA3.1 (Search Vector Database) Backbone size w/o insert (bp) 5345 Vector type Mammalian Expression Selectable markers Neomycin (select with G418) Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name …

NFkB 转录响应元件（TRE）克隆 易锦生物 iGeneBio

WebHaloTag® Fusion (N-Terminal) Mammalian Expression Vectors Inducible T7-Driven Flexi® Vectors for E. coli Expression このベクターは哺乳類細胞においてC末端にHaloTag®を … WebpMH-Halo tag (Search Vector Database) Backbone size (bp) 7412 Vector type Mammalian Expression Selectable markers Neomycin (select with G418) Tag / Fusion Protein Halo tag (N terminal on backbone) Growth in … swimming pool kits uk https://rhinotelevisionmedia.com

pHTC HaloTag® CMV-neo Vector - Promega

WebApr 14, 2024 · Full-length SNORD44 was cloned into the pBluescript vector, and both the sense and anti-sense SNORD44 sequences were transcribed in vitro. ... 293 T cells constitutively expressing the Halo tag ... WebHaloTag®技术 ; shRNA相关技术 ... House keeping gene promoter positive control in single-reporter vector: 875 . WebJun 1, 2016 · I found Halo-tag vectors from Promega. They have many versions of the vector (all have C-terminal Halo).pFC14A HaloTag® CMV Flexi® Vector expresses the high level of recombinant but does... swimming pool ktm

NFkB 转录响应元件（TRE）克隆 易锦生物 iGeneBio

Category:pFC14A and pFC14K HaloTag® CMV Flexi® Vectors

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Halo tag vector

pHTC HaloTag® CMV-neo Vector - Promega

WebpFN28A HaloTag™ CMV-neo Flexi™ vector: Each for $320.50 Add to cart PRG8451 View Documents View Product Certificates. Promega G8451 0.1 μg/μL: 10mM Tris HCl, 1mM EDTA (pH 8) pFN28K HaloTag™ CMV-neo Flexi™ vector ... WebpFN28A HaloTag™ CMV-neo Flexi™ vector: Each for $320.50 Add to cart PRG8451 View Documents View Product Certificates. Promega G8451 0.1 μg/μL: 10mM Tris HCl, 1mM …

Halo tag vector

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WebAll clones were generated using Promega's pIX-HALO vector which confers ampicillin resistance and are distributed in E. coli host strain DH5alpha-TIR. The pIX-HALO vector is a custom-built in vitro expression vector suitable for protein expression in both wheat germ lysate and rabbit reticulocyte expression systems.

WebA vector was constructed which expresses the HaloTag protein under the control of the 35S promoter of cauliflower mosaic virus. The functionality of the HaloTag construct was … WebすべてのHaloTag® Vectorを見る HaloTag® Fusion (N-Terminal) Mammalian Expression Vectors Inducible T7-Driven Flexi® Vectors for E. coli Expression. このベクターは哺乳類細胞においてC末端にHaloTag®を融合したタンパク質を発現させるためにデザインされまし …

WebThe constructed vector was transformed into Rosetta 2 (DE3)-competent cells (Sigma-Aldrich). ... In another study using mouse embryonic stem cells (mESCs) expressing endogenous RAD21 with a Halo-tag, 109,400 RAD21 molecules per cell were estimated . Although it is difficult to make a strict comparison between the amounts of cohesin in … WebThe HaloTag® fusion vectors are designed to be used as the fluorescent acceptor in NanoBRET™ assays that monitor interactions of a specific target protein along the UPS pathway. NanoBRET™ technology is a …

WebNov 11, 2024 · Halo Tags. A Halo tag is a file that stores specific kinds of information, grouped by type. For example, a particular kind of vehicle in the game will have its data …

WebpFN18A HaloTag T7 Flexi® vector with an ampicillin resistance marker, for bacterial or cell-free expression of a protein with a cleavable N-terminal HaloTag®. Sequence Author: Promega Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7kPlasmids:Basic Cloning Vectors More Plasmid Sets … swimming pool kreepy krauly cleanerWeb• Labelling of cells with particular HaloTag ligand • Formation of a vector that encodes the fusion of HaloTag protein to a protein or protein-domain [6]. When enzyme and particular ligand come in contact, formation of an alkyl-enzymeintermediatetakes placewhennucleophilic displacement of terminal swimming pool kool deck paintWebThis system allows different fluorescent colours to be used to visualize the localization of the non-fluorescent HaloTag protein within living cells. A vector was constructed which expresses the HaloTag protein under the control of … swimmingpool kunststoff