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H9c2 cell transfection

WebOct 14, 2024 · Interestingly, we also found that transfection of H9c2 cells with a Gfi-1 overexpression plasmid reduced TNF-α and IL-6 levels and downregulated the expression of NF-κB and TNF-α in H9c2 cells at 6 h and 24 h after LPS induction in the LPS + Gfi-1 group (Fig. 2A–C). These data strongly demonstrate that Gfi-1 plays a protective role in LPS ... WebMar 2, 2024 · H9c2 cells were lysed in RIPA lysis buffer (Epizyme Biotech, Shangai, China) and centrifuged at 12,000 ... On the other hand, transfection of miR-31-5p mimic in H9c2 cells led to decreased levels of circPan3 compared with the control (Fig. 4G). Together, these results indicated that miR-31-5p is involved in DOX-induced cardiotoxicity via the ...

Are harshly split H9C2 cells still viable for transfection?

WebLipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. The reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to ... WebAug 21, 2024 · Cell Transfection and Exosome Expression in 3D Hydrogel Culture: For these experiments, H9c2 cells (10{circumflex over ( )}6) are seeded onto PEG-fibrinogen hydrogels (5×5×1 cm rectangular prism) immersed in DMEM with 10% FBS and incubated at 37 Celsius for 24 hours. flu clinic island health https://rhinotelevisionmedia.com

The inhibition of miR‑101a‑3p alleviates H/R injury in H9C2 cells …

WebH9c2 Transfection Reagent (Embryonic Myocardium) $ 326.00 – $ 1,794.00 SKU: H9C2 Product Availability: In Stock (FedEx delivery within 2 business days). Ordering: To place an order please use Add to Cart … WebThe reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively … WebDec 17, 2024 · H9c2 cells were transfected in 24-well plates with miR-29b-3p mimic and wild-type SPARC-3’-UTR or miR-29b-3p mimic and mutant SPARC-3’-UTR respectively using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. flu clinic hamilton

h9c2 Cells Thermo Fisher Scientific - US

Category:Microneedle array electroporation system for cell transfection

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H9c2 cell transfection

LPS-aggravated Ferroptosis via Disrupting Circadian …

WebBased on our proteomic analysis, its presence in EVs was linked to hypoxia in HL1 and H9c2 cells, and we hypothesize that EV-associated ENPL may have a cardioprotective role by reducing cardiomyocyte ER stress. ... Transfection of H9c2 and HL1 Cells with pMAXGFP-ENPL Plasmid. H9c2 and HL1 cells were cultured up to 80% confluency in … Webtransfection efficiency; however it may increase cell toxicity 6. Add prepared transfection complexes to 0.5 ml of complete growth medium with H9C2 cells (from step 2) 7. …

H9c2 cell transfection

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WebJun 29, 2015 · H9c2 myoblasts are a cell model used as an alternative for cardiomyocytes. H9c2 cells have the ability to differentiate towards a cardiac phenotype when the media serum is reduced in the presence of all-trans-retinoic acid (RA), creating multinucleated cells with low proliferative capacity. In the present study, we performed for the first time … WebJan 13, 2015 · CELL TRANSFECTION IN HEK-293 and H9c2 (2-1) cell lines In each well of a 6-well plate, seed either HEK-293 or H9c2 (2-1) 5x105 cells in 4ml of complete growth …

WebMar 1, 2011 · Expression of exogenous DNA in vitro is significantly affected by the particular transfection method utilized. In this study, we evaluated the efficiency of two transfection methods, chemically mediated polyethyleneimine (PEI) treatment and physically mediated electroporation, on a rat heart myoblast cell line, H9c2(2-1). After PEI transfection of … WebApr 13, 2024 · Circadian disruption is involved in the progress of sepsis-induced cardiomyopathy (SICM), one of the leading causes of death in sepsis. The molecular …

WebAdd 1μg of plasmid DNA to the medium, and mix. For a 3:1 ViaFect™ Transfection Reagent:DNA ratio, add 3μl of ViaFect™ Transfection Reagent, and mix immediately. Incubate the ViaFect™ Transfection Reagent:DNA mixture for 5–20 minutes at room temperature. Optional: Add mixture to cells without an incubation period. WebGrowing H9C2 cells and trying to ensure they maintain the phenotye of Cardiac cells. They are grown in T150 flasks, maintained and grown in 25ml of MEM (containing 10% FCS, Na+ Pyruvate,...

WebAug 30, 2024 · Protocatechuic acid reduced isoproterenol-induced cardiomyocyte hypertrophy in H9c2 cells. ... Small interfering RNAs (siRNA) transfection. H9c2 cells were transfected with 100 nM control siRNA ...

WebApr 12, 2024 · After transfection and hydrogen peroxide stimulation, H9C2 cells were fixed with 4% paraformaldehyde and cells were incubated with 50 μg/ml WGA for 30 min at 37 °C. Images were observed by fluorescence microscopy. Surface area was measured with ImageJ and the value was expressed as fold change relative to control. flu clinic massachusettsWebMar 1, 2016 · What is the recommended seeding density for H9C2 cells (specifically in a plastic tc t25 flask)? We have been seeding at the recommended 10x10^4/cm^2 (250,000 in t25) and they are reaching ~70%... green earth tree service houston texasWebJul 16, 2024 · H9C2 cells (ProCell, Wuhan, China) were subcloned from BD1X rat embryonic heart tissue and cultured in Dulbecco’s modified Eagle medium supplemented … flu clinic nelson bc