WebOct 14, 2024 · Interestingly, we also found that transfection of H9c2 cells with a Gfi-1 overexpression plasmid reduced TNF-α and IL-6 levels and downregulated the expression of NF-κB and TNF-α in H9c2 cells at 6 h and 24 h after LPS induction in the LPS + Gfi-1 group (Fig. 2A–C). These data strongly demonstrate that Gfi-1 plays a protective role in LPS ... WebMar 2, 2024 · H9c2 cells were lysed in RIPA lysis buffer (Epizyme Biotech, Shangai, China) and centrifuged at 12,000 ... On the other hand, transfection of miR-31-5p mimic in H9c2 cells led to decreased levels of circPan3 compared with the control (Fig. 4G). Together, these results indicated that miR-31-5p is involved in DOX-induced cardiotoxicity via the ...
Are harshly split H9C2 cells still viable for transfection?
WebLipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. The reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to ... WebAug 21, 2024 · Cell Transfection and Exosome Expression in 3D Hydrogel Culture: For these experiments, H9c2 cells (10{circumflex over ( )}6) are seeded onto PEG-fibrinogen hydrogels (5×5×1 cm rectangular prism) immersed in DMEM with 10% FBS and incubated at 37 Celsius for 24 hours. flu clinic island health
The inhibition of miR‑101a‑3p alleviates H/R injury in H9C2 cells …
WebH9c2 Transfection Reagent (Embryonic Myocardium) $ 326.00 – $ 1,794.00 SKU: H9C2 Product Availability: In Stock (FedEx delivery within 2 business days). Ordering: To place an order please use Add to Cart … WebThe reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively … WebDec 17, 2024 · H9c2 cells were transfected in 24-well plates with miR-29b-3p mimic and wild-type SPARC-3’-UTR or miR-29b-3p mimic and mutant SPARC-3’-UTR respectively using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. flu clinic hamilton