http://www.hitechglobal.com/FMCModules/FMC+FireFly.htm WebNov 18, 2015 · U87 glioblastoma cells, which endogenously express predominantly NOTCH2 but only very low levels of other NOTCH receptors, were co-transfected with a Notch-responsive TP-1 (12X CSL) firefly ...
Notch-Delta coculture assay.
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WebMay 27, 2011 · CTL and shAAK1 HeLa cell lines were transiently transfected with CSL-luciferase reporter ( 38, 39 ), pRL-TK (Promega) and Notch ΔE. Luciferase-assay was performed 24 h post-transfection using the dual-luciferase kit (Promega) following the manufacturer's instructions using a Berthold luminometer. Coculture Assay WebDownload scientific diagram Notch-Delta coculture assay. Modulation of the Notch reporter activity (CSL-luciferase) in Notch1-expressing cells cocultured with Dll1-expressing cells (Lanes B–N ... WebFirefly makes it easy for schools to share information, helps parents support student achievement and engages everyone in the learning conversation, without adding to the … billy madison movie teacher