Cisplatin dna cross-linking

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WebRecent studies have shown that the majority of proteins cross-linked to DNA by cisplatin in situ are nuclear matrix proteins ( 2 - 4 ). We have also shown that cisplatin cross-links … WebFeb 21, 2000 · Most simple cis-PtA2G2 complexes that model the G-G cross-link DNA lesions caused by the clinically used anticancer drug cis-PtCl2 (NH3)2 undergo large fluxional motions at a rapid rate (A2 = two amines or a diamine; G = guanine derivative).

Solution structure of a DNA duplex containing a cis …

WebThis chapter deals with the interstrand cross-links in cisplatin- or transplatin-modified DNA and focuses on three main aspects: the formation of the interstrand cross-links, the … Webwww.ncbi.nlm.nih.gov can seafood make you sick

Isolation of Proteins Cross-linked to DNA by Cisplatin

WebCisplatin Medicine & Life Sciences 74%. DNA Repair Medicine & Life Sciences 50%. DNA Medicine & Life Sciences 46%. DNA Adducts Medicine & Life Sciences 34%. ... (II) and Modification of Platinum-induced DNA Interstrand Cross-Linking. / Swinnen, Lode J.; Barnes, Diane M.; Fisher, Susan G. et al. WebJul 8, 2009 · The two opposing guanine residues involved in the cross-link were identified via limited alkaline hydrolysis. The kinetics of aquated cisplatin binding to the BBD RNA, a related RNA hairpin, and its DNA hairpin analogue were investigated in an ionic background of 0.1 M NaNO (3) and 1 mM Mg (NO (3)) (2). WebFeb 1, 2002 · To overcome this obstacle, researchers have focussed their attention on cisplatin (cis-DDP; cis-platinum (II)diamminedichloride), a cross-linking agent shown to … flannel pajamas for women amazon

Laser-Induced Photo-Cross-Linking of Cisplatin-Modified …

Cisplatin 14. DNA - Chemistry LibreTexts

WebApr 14, 2024 · Among the extracellular vesicles, apoptotic bodies (ABs) are only formed during the apoptosis and perform a relevant role in the pathogenesis of different diseases. Recently, it has been demonstrated that ABs from human renal proximal tubular HK-2 cells, either induced by cisplatin or by UV light, can lead to further apoptotic death in … WebThe DNA binding is similar to cisplatin, and the complex forms a highly stereoselective adduct on DNA, but not for reactions with mono- and dinucleotides. 98 In cell lines with previously determined mechanisms of cell resistance, AMD473 showed little cross-resistance compared with cisplatin or carboplatin. flannel pajamas bottoms elastic waist mensWebROS can induce single or double-strand breakage, nucleotide alterations, deoxyribose changes, and DNA cross-linking in DNA. If oxidative DNA damage is not repaired before DNA replication, it could lead to cell death, DNA mutation, replication mistakes, and genomic instability (Cooke et al., 2003; Schupp et al., 2016). flannel pajamas for women near me

"WebThis chapter deals with the interstrand cross-links in cisplatin- or transplatin-modified DNA and focuses on three main aspects: the formation of the interstrand cross-links, the distortions induced in the DNA double helix and the potential use of the interstrand cross-linking reaction in the context of antisense and antigene strategies. " - Cisplatin dna cross-linking

Cisplatin dna cross-linking

Influence of cisplatin intrastrand crosslinking on the ... - PNAS

WebFeb 1, 2002 · To overcome this obstacle, researchers have focussed their attention on cisplatin (cis-DDP; cis-platinum (II)diamminedichloride), a cross-linking agent shown to crosslink protein to DNA and... WebJan 1, 2001 · We compared the DNA cross-linking capability of CC-1065 with that of its analogs: Bizelesin, which forms DNA cross-links by direct chemical bonding, and Adozelesin, a demethoxy derivative of CC-1065. Section snippets Drugs

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Webfavors cisplatin cross-linking (20) with inter- and intra-DNA strands (14,17,26). Cisplatin interacts primarily with cytosine- and guanine-rich DNA regions (29) and causes cellular damage. Despite its potent antineoplastic action, cisplatin’s major side effects include nephrotoxicity (2), peripheral neuropathy (33), azoospermia (11). In mammals, WebMar 15, 2007 · DNA–protein cross-linking by cisplatin or transplatin. Cisplatin and transplatin were investigated for their ability to form ternary DNA–protein complexes …

WebCisplatin produces DNA cross-linking and is used to treat medulloblastoma, head and neck, ovarian, germ cell, cervical, lung and bladder cancers. Cisplatin produces a … WebJul 23, 1996 · Abstract cis-Diamminedichloroplatinum (II) (cisplatin) is a widely used anticancer drug that binds to and crosslinks DNA. The major DNA adduct of the drug results from coordination of two adjacent guanine bases to platinum to form the intrastrand crosslink cis- [Pt (NH3)2 [d (GpG)-N7 (1), -N7 (2)]] (cis-Pt-GG).

WebPhoto-cross-linking is thus an effective tool for investigating the interaction of cisplatin-modified DNA with damage-recognition proteins under heterogeneous conditions such those in cell extracts or living cells. † Supported by Grant … WebSep 28, 2012 · DNA cross-linking agents such as the nitrogen mustards (melphalan, chlorambucil, cyclophosphamide, ifosfamide), platinum drugs (cisplatin, carboplatin, oxaliplatin), chloroethylnitrosoureas (carmustine, lomustine), the alkylalkane sulphonate busulphan and the natural product mitomycin C are widely used drugs as both single …

WebNov 15, 1987 · Two sublines were derived from a human bladder carcinoma continuous cell line (RT112-P), one by exposure to fractionated X-irradiation (RT112-DXR8) and the other by continuous exposure to cisplatin (RT112-CP). RT112-DXR8 cells were 1.6- to 2-fold more sensitive to cisplatin and 2 analogues, carbopla …

WebWe evaluated the growth and molecular interactions of the anti-EGFR antibody cetuximab (erbitux) and the DNA cross-linking agent cisplatin (cis-diammedichloroplatinum; CDDP) in the gefitinib-resistant MDA-MB-468 breast cancer cell line, an in vitro model system that shows many of the recurrent basal-like molecular abnormalities including … can seagulls drink salt waterWebCisplatin is a platinum-based drug which acts by binding to DNA by forming intrastrand cross-link conjugate which eventually leads to apoptosis due to the activation of p53, … flannel pajamas for men at walmartWebCisplatin induces DNA damage through inter-strand or intra-strand cross-linking of DNA . Its dose-limiting toxicity is nephrotoxicity, peripheral nerve toxicity, and toxicity to the cochlea (the inner ear toxicity) . Paclitaxel is a microtubule poison that … can seagulls fartWebCisplatin crosslinks DNA in several different ways, interfering with cell division by mitosis. The damaged DNA elicits DNA repair mechanisms, which in turn activate apoptosis when repair proves impossible. flannel pajamas for women floralWebDec 15, 1995 · The widely used antitumor drug cis -diamminedichloroplatinum (II) (cisplatin or cis -DDP) reacts with DNA, cross-linking two purine residues through the N7 … can seagrass rugs get wetWebJun 24, 2011 · For example, cisplatin, the most widely used crosslinking drug, causes damage that is estimated to comprise of 90% intrastrand crosslinks (predominantly crosslinks between adjacent purine... flannel pajamas for men with flyWebAs we have seen from studies into the mode of action of cisplatin, covalent intrastrand cross-links can also be formed by chemical agents, such as cisplatin. A similar mechanism is believed to repair damage done to cancer cells by cisplatin, as we will see when we discuss drug resistance. can seagate external hard drive be detected